Abstract
P20778, an Indian strain of Japanese encephalitis virus (JEV) obtained from Vellore in the Southern India, was grown in Vero cells cultured on microcarriers in a spinner flask. The virus was formalin-inactivated and its immunogenicity and protective efficacy in mice were tested in comparison with a commercially available vaccine. Our studies indicated that formalin-inactivated JEV P20778 induced high levels of protective immunity in mice. Virus inactivation with formalin at 22 °C, which required shorter incubation period, was found to be as good or better to virus inactivation at 4 °C for generating high titers of anti-JEV antibodies. Similarly, the 22 °C-inactivated virus generated JEV neutralizing antibody titers as good or higher than those induced by the 4 °C-inactivated virus. Thus, for the vaccine production, inactivation of JEV with formalin at 22 °C would be a preferred method as it is faster and does not require cold room storage.
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