IMMUNOGENICITY AND EFFICACY OF BACULOVIRUS DERIVED GLYCOPROTEIN D OF HERPES SIMPLEX VIRUS TYPE-1 IN MICE

Abstract

Summary The recombinant glycoprotein D (gD) of herpes simplex virus type-1 (HSV-1) in baculovirus expression system was produced. Spodoptera Frugiperda cell, clone 9 (Sf9) was cultured in modified Grace's medium and inoculated with 57 multiplicity of infection of HSV-1 recombinant baculovirus carrying gD gene. Inoculated cells were harvested 96h postinoculation and treated with 3[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) and phenylmethylsulfonyl fluoride (PMSF) and sonicated at 20KHZ for 5 times. A western blotting was developed and applied to detect the prepared protein. Three groups of BALB/c mice each of 7 mice were inoculated with the recombinant gD, sublethal dose of challenge virus (10 4.5 TCID50) and PBS respectively. All inoculated mice were challenged with 10MLD50 (10 6.5 TCID50) of the wild HSV-1. All mice who had received the recombinant gD survived while 14.3% and 71.5% of mice inoculated respectively with either sublethal dose of the virus or PBS died.