Abstract
The aim of the study was to investigate immunogenic properties of mosaic recombinant proteins constructed on the data of hepatitis C virus NS4A and NS4B antigens. Four mosaic recombinant proteins, containing the T and B epitopes of the NS4A and NS4B antigens, were created by genetic engineering methods in the E. coli system. To enhance the immune response they were linked in different variations to the nucleotide sequences of murine interleukin-2 (IL-2), the Neisseria meningiditis lipopeptide, and the T helper epitope of the core protein of hepatitis C virus. The immunogenic properties of these recombinant proteins were analyzed by immunoblotting, ELISA and ELISpot using sera from immunized mice and patients infected with hepatitis C virus. Recombinant proteins specifically reacted with the sera of immunized mice and infected patients in immunoblotting. According to the ELISA data, the predominant formation of antibodies to NS4B was observed when mice were immunized with the recombinant proteins containing both antigens. Analysis of gamma-interferon production by T-lymphocytes upon contact with activated dendritic cells showed in ELISpot that the maximum production of this cytokine was detected when adjuvant components were located at the N- and C-ends of the recombinant protein. The highest level of gamma-interferon production during stimulation with this drug was detected in lymphocytes from the bone marrow and lymph nodes. The recombinant protein containing the T and B epitopes of NS4A and NS4B, murine IL-2 and the lipopeptide Neisseria meningiditis had the greatest immunostimulate effect among the four constructions. This recombinant protein formed nanoparticles of 100-120 nm in size.
Highlights
Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus
The aim of the study was to investigate immunogenic properties of mosaic recombinant proteins constructed on the data of hepatitis C virus NS4A and NS4B antigens
Важное сугубо эпидемиологическое значение имеетОбъёмное изображение наночастиц препаратов LTNb (а) и LTNb-NS4a-IL2 (б) по данным АСМ
Summary
5. Tseng C.H., Knowles N.J., Tsai H.J. Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. Classification duck hepatitis virus in to three genotypes bases on molecular evolutionary analysis. 7. Vinokhodov O.V., Vinokhodov V.O., Vinokhodov D.O. Enterovirus hepatitis of ducks of I type. Viral Hepatitis of Birds [Virusnye gepatity ptits]. Temperature and pH stability of duck hepatis virus. Knyazev V.P. Viral hepatitis of ducks (ducks). Syurin V.N., Belousova R.V., Fomina N.V. Veterinary Virology [Veterinarnaya virusologiya]. Ulupov N.A., Mikhalishin V.V., Gusev A.A., Lezova T.A. Aminoethylethylenimine is a means of inactivating the infectivity of the foot and mouth disease virus. Borisova I.A. Antigenic activity of experimental inactivated emulsion vaccine against Newcastle disease and pneumovirus infection of birds. Куприянов В.В.1, Николаева Л.И.2, Зыкова А.А.1, Махновский П.И.2, Котляров Р.Ю.1, Васильев А.В.2, Равин Н.В.1
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