Immunogenic evaluation of a recombinant 49-kilodalton outer membrane protein of Salmonella typhi as a candidate for a subunit vaccine against typhoid

Abstract

Typhoid poses a global public health concern. Presently available vaccines against typhoid have certain drawbacks that make them unsuitable for mass immunization, especially to children and elderly people. The outer membrane proteins (OMPs) of Salmonella have strong potential for the development of a candidate subunit vaccine against typhoid. We previously reported that a 49 kDa OMP confers protection against experimental salmonellosis. In the present study we report the cloning, expression of a recombinant protein to evaluate its potential to elicit an immune response against a challenge with Salmonella. The gene encoding the 49 kDa