Immunofluorescent localization of dopamine-like and leucine-enkephalin-like neurons in the supraoesophageal ganglia of the American cockroach, Periplaneta Americana

Abstract

Leucine-enkephalin- and dopamine-like nerve cells and fibers were localized in the supraoesophageal ganglia (brain) of the American cockroach, Periplaneta americana, using immunofluorescence. The presence of leucine-enkephalin-like material was confirmed using immunoperoxidase staining. Several cells containing leucine-enkephalin-like material were found in the pars lateralis, and nerve fibers belonging to these cells were traced through the brain. Dopamine-like material was detected in deutocerebral neurons as well as the nerve processes arising from these cells which lead into the area of the deutocerebral glomeruli. Specific immunofluorescence was also obtained in the alpha and beta lobes of the corpora pedunculata with both the leucine-enkephalin and dopamine antibodies. However, the fluorescent banding pattern observed in both lobes was distinctly different with the two antibodies. No specific fluorescence was observed in the stalk or peduncle of the corpora pedunculata with either the leucine-enkephalin or the dopamine antibody. The findings suggest a possible interaction of leucine-enkephalinergic and dopaminergic nerve fibers in the alpha and beta lobes of the cockroach corpora pedunculata.