Abstract
Specific immunofluorescence staining for AFP of the rat yolk sac showed that the synthesis was confined to the visceral layer of the yolk sac endoderm. The AFP synthesis took place not only in the extraplacental yolk sac as demonstrated by Gitlin in 1967, but also in the endodermal sinus structures within the placenta. No immunofluorescence staining was found of Reichert's membrane close to the parietal layer of the yolk sac. These findings are in accordance with the earlier demonstrated AFP synthesis in the visceral layer of the endodermal sinus structures in the tumors.
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