Abstract
DIFFUSION chamber cultivation studies with human lymph-node tissues have shown a high proliferative activity of the immune cell population immediately before and during antibody production as indicated by the incorporation of tritiated thymidine in vitro1. It became of interest, therefore, to determine the proliferative capacity of the antibody-containing cells as compared with the cells in the same population which were not forming antibody. This was done by using both in vitro and in vivo exposure of the cultivated cells to tritiated thymidine and subsequent staining of the cells for antibody by immunofluorescence followed by autoradiography.
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