Abstract
Background. Class III beta-tubulin (TUBB3) is one of the eight beta tubulin isotypes identified in human. It is constitutively expressed in brain and testis but not in lung tissue. TUBB3 is also known to appear in solid tumors, in particular in non-small cell lung cancer (NSCLC), and it is often associated with poor prognosis and resistance to taxanes and Vinka alkaloids. Objective: We have suggested that TUBB3 expression may cover not only the primary tumor but also a wide adjacent area of morphologically normal lung tissue. To check the hypothesis we have measured TUBB3 expression both in the non-small cell lung carcinoma (NSCLC) and remote lung tissue derived from the same lung. 60surgical biopsy specimens of NSCLC and morphologically normal lung tissue of 30patients were investigated. Materials and methods. Biopsy specimens were converted to suspension, fixed in 4 % formaldehyde and analyzed by flow cytometry using monoclonal antibodies to TUBB3. The method was developed in the laboratory of medicinal chemistry research Institute of experimental diagnostics and therapy of tumors (N.N. Blokhin Russian Cancer Research Center) and patented. Results. Fraction of TUBB3-positive cells in the group of adjacent lung tissue specimens was lower compared to NSCLC group but still positive in the majority of adjacent lung tissue specimens (25 of 30) and achieved up to 39 % of cells. Conclusion. We suggest that TUBB3 expression in adjacent morphologically normal lung tissue indicates presence of transformed and potentially malignant cells far from the primary site.
Highlights
Class III beta-tubulin (TUBB3) is one of the eight beta tubulin isotypes identified in human
TUBB3 is known to appear in solid tumors, in particular in non-small cell lung cancer (NSCLC), and it is often associated with poor prognosis and resistance to taxanes and Vinka alkaloids
We have suggested that TUBB3 expression may cover the primary tumor and a wide adjacent area of morphologically nor mal lung tissue
Summary
Блохина» Минздрава России; Россия, 115478, Москва, Каширское шоссе, 24. В то же время экспрессия TUBB3 выявляется в клетках солидных опухолей, в частности немелкоклеточного рака легкого (НМРЛ), где его экспрессию обычно связывают с неблагоприятным прогнозом и устойчивостью к терапии препаратами платины и таксанами. Исследовано 60 хирургических биопсийных образцов немелкоклеточного рака легкого и морфологически нормальной окружающей ткани 30 пациентов, оперированных в ФГБУ «РОНЦ им. Экспрессия TUBB3 в группе образцов НМРЛ была выше, чем в группе образцов морфологически нормальной ткани. Тем не менее в подавляющем большинстве образцов морфологически нормальной ткани (25 из 30) TUBB3 экспрессировался, достигая значения в 39 % окрашенных клеток. В целом, полученные данные свидетельствуют о принципиальной возможности присутствия TUBB3-позитивных клеток за пределами первичной опухоли – в ткани, визуально не вовлеченной в опухолевый процесс. Ключевые слова: немелкоклеточный рак легкого (НМРЛ), нормальная ткань легкого, бета-III тубулин (TUBB3), проточная цитофлуориметрия. Blokhin Russian Cancer Research Center, Ministry of Health of Russia; 24 Kashyrskoe shosse, Moscow, 115478, Russia
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