Abstract
Sensitive and rapid detection of pathogenic bacteria plays an important role in avoiding food poisoning. However, the practical application value of conventional assays for detection of foodborne bacteria, are limited by major drawbacks; these include the laboriousness of pure culture preparation, complexity of DNA extraction for polymerase chain reaction, and low sensitivity of enzyme-linked immunosorbent assay. Herein, we designed a non-complex strategy for the sensitive, quantitative, and rapid detection of Salmonella typhimurium with high specificity, using an anti-Salmonella typhimurium IgG-AuNC-based immunofluorescent-aggregation assay. Salmonella typhimurium was agglutinated with fluorescent anti-Salmonella typhimurium IgG-AuNC on a glass slide, and observed using a fluorescence microscope with photoexcitation and photoemission at 560nm and 620nm, respectively. Under optimized reaction conditions, the AuNC-based immunofluorescent-aggregation assay had a determinationrange between 7.0 × 103 and 3.0 × 108CFU/mL, a limit of detection of 1.0 × 103CFU/mL and an assay response time of 3min. Thetechnique delivered good results in assessing real samples.
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