Abstract
Abstract Techniques were described for the cultivation and immunofluorescent identification of Hemophilus influenzae, Diplococcus pneumoniae, Neisseria meningitis, and eight less common pathogens in specimens of cerebrospinal fluid from 100 patients with bacterial meningitis. A comparison of the results obtained by conventional methods and by immunofluorescent staining indicated that the latter method was fully an sensitive as the former and was more accurate in treated cases. Some of the dangers involved in the use of the Gram stain of the sediment as a tool for presumptive diagnosis were discussed, as were shortcomings of fluorescent antibody staining, particularly in infections caused by uncommon gram-negative organisms. The immunofluorescent staining technique was recommended for the rapid screening of spinal fluid specimens, as well as of cultural isolates.
Published Version
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