Abstract
The ability of methylene blue to photooxidize the guanine moiety of intracellular DNA of living human cells was demonstrated by immunofluorescence using an antibody with a specificity for the unpaired cytosine residue of DNA. These findings which indicate that sufficient amounts of the dye penetrate the mammalian cell to cause biological effects may present the investigator with the unique opportunity of selectively altering the guanine residue in DNA and thereby determining the genetic consequences of this reaction.
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