IMMUNOEXPRESSION OF H3K9AC IN DENTAL FOLLICLE AND AMELOBLASTOMA

Abstract

Histone modification, such as histone 3 lysine 9 acetylation (H3K9ac), is a major posttranslational epigenetic mechanism related to physiologic process of repairing, replication, and gene expression. Changes in H3K9ac immunoexpression have been recently reported in some human pathologies, for example in oral squamous cell carcinoma, but it has not yet been described in ameloblastoma. Objectives: To report H3K9ac immunoexpression in ameloblastoma, correlating it with dental follicles. Study Design: Ten dental follicles and 38 ameloblastoma cases with 3-µm sections in silanized slides underwent immunohistochemical reactions for H3K9ac antibody (C5B11 – Cell Signaling) in order to determine the immunoexpression profile, mean positivity and staining intensity. All samples were digitalized into high-resolution images using the Aperio Scanscope CS Slide Scanner and evaluated using software for digital analysis. Results: The respective mean values of positivity and intensities (weak, medium and strong) were 71.79%, 17.93%, 38.60%, and 15.26% for dental follicles, whereas 67.84%, 18.69%, 34.08%, and 15.07% for ameloblastoma. Conclusions: Although other human pathologies show H3K9ac immunoexpression changes in relation with normal tissues, the ameloblastoma development and progression may be not correlated with epigenetic changes involving histone 3 acetylation. Histone modification, such as histone 3 lysine 9 acetylation (H3K9ac), is a major posttranslational epigenetic mechanism related to physiologic process of repairing, replication, and gene expression. Changes in H3K9ac immunoexpression have been recently reported in some human pathologies, for example in oral squamous cell carcinoma, but it has not yet been described in ameloblastoma. Objectives: To report H3K9ac immunoexpression in ameloblastoma, correlating it with dental follicles. Study Design: Ten dental follicles and 38 ameloblastoma cases with 3-µm sections in silanized slides underwent immunohistochemical reactions for H3K9ac antibody (C5B11 – Cell Signaling) in order to determine the immunoexpression profile, mean positivity and staining intensity. All samples were digitalized into high-resolution images using the Aperio Scanscope CS Slide Scanner and evaluated using software for digital analysis. Results: The respective mean values of positivity and intensities (weak, medium and strong) were 71.79%, 17.93%, 38.60%, and 15.26% for dental follicles, whereas 67.84%, 18.69%, 34.08%, and 15.07% for ameloblastoma. Conclusions: Although other human pathologies show H3K9ac immunoexpression changes in relation with normal tissues, the ameloblastoma development and progression may be not correlated with epigenetic changes involving histone 3 acetylation.