Abstract

A new highly sensitive method of measuring cytokinin oxidase activity was worked out on the basis of an immunoenzyme test-system for isopentenyladenosine (IPA) assay. The enzyme activity is determined by immunoenzyme assay as the difference between the IPA quantities in the reaction mixture before and after incubation. The specificity of the assay was verified by using the well-known enzyme activators and inhibitors and by monitoring the formation of other cytokinins.

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