Abstract
Infection of green bamboo (Bambusa oldhamii Munro), with bamboo mosaic virus (BoMV), a member of the potexvirus group, results in the formation of a large number of unusual electrondense crystalline bodies (EDCB), in addition to virion aggregates, in the infected cells. In order to determine the subcellular site(s) of sequential appearance of EDCB and capsid protein in the infected cells, the infection sequence was followed in leaf tissues. Emerging young leaves of green bamboo that have not yet unrolled were sampled about 2 weeks post-inoculation. Leaf tissues were fixed with glutaraldehyde and embedded in Lowicryl HM20. Ultrathin sections were first treated with 1:200 dilution of anti- BoMV serum followed by gold-labelled goat anti-rabbit IgG.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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