Abstract
Elastic tissue is recognised to be composed of a major amorphous component (consisting of the protein elastin) and a lesser microfibrillar component (complex structures containing at least two glycoproteins). The study of the components of elastic fibers in developing tissues has been hampered by the difficulty of establishing that the amorphous component is distinguishable from other amorphous structures. This is particularly a problem when the elastic material is deranged, so that the amorphous component is atypical in appearance. We have developed, in rabbits, and then affinity-purified, polyclonal antibodies to each of elastin (the amorphous component), “reductive guanidine extracts” of fetal bovine nuchal ligament and to a microfibril-associated glycoprotein (MAGP) isolated from these extracts.Samples of fetal and adult bovine tissues, including aorta, skin and nuchal ligament; and human tissues; including skin, aorta and breast cancers, were fixed for 4 h in 4% paraformaldehyde and 0.2% glutaraldehyde in phosphate buffered saline, pH 7.4, containing 4% sucrose and 5% polyvinyl pyrolidine;
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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