Immunoelectron microscopical demonstration of endogenous avidin in secretory granules of the hen oviduct mucosa: a preliminary study.

Abstract

The localization of avidin in the oviduct of the laying hen was investigated using ultrastructural immunoperoxidase techniques. Endogenous avidin was localized in secretory granules of both tubular gland cells and non-ciliated single epithelial cells in the magnum mucosa. These immunospecific granules were electron-dense and heterogeneous with a patchy core and dense peripheral region, especially in acinar cells. The size varied from small to large in the gland cells (500-2200 nm in diameter) and remained small in the epithelial cells (180-720 nm). Columnar epithelial cells containing avidin granules strongly resembled the protodifferentiated tubular gland cells appearing in the magnum mucosa of chicks artificially pretreated with ovarian hormones. On the other hand, no avidin was observed in either epithelial goblet cells or ciliated cells in adult hens, although both cell types were shown to produce avidin in young chicks when synchronized by the administration of progesterone. The present results parallel those obtained with biotinylated enzyme affinity methods in our previous cytochemical study. Therefore, avidin is one of the proteins produced and stored in the secretory granules of the tubular gland cells and protodifferentiated acinar cells present in the epithelial layer of the laying hen oviduct. It is not present in goblet cells. Although the initiation of a synthesis may be triggered by progesterone, it is still not clear whether different hormone dependent proteins are localized in the same granules in both the adult hen and the immature chick.