Immunoelectron microscopic localization of Rous sarcoma virus structural protein p27 in infected chicken embryo fibroblasts.

Abstract

The immunoperoxidase technique was employed to localize, at the ultrastructural level, protein Pr76 (a precursor of the 'gag' gene protein of avian leukemia and sarcoma viruses) in chick embryo cells infected with RSV-RAV-2 virus. The protein was confined to free and membrane-bound ribosomes. This was often particularly conspicuous in the perinuclear region. No staining was found in the ergastoplasmic cisternae, in the Golgi apparatus, or in the nucleus. This pattern of localization is compatible with previous studies where the protein was searched for in various cell subfractions.