Abstract

Klebsiella capsular antigens K1-K72 and O antigens O1-O9 were isolated and rabbits immunized with corresponding bacteria. The immunoelectrophoretic patterns of the O and K antigens were studied. All K antigens analyzed were highly electrophoretically mobile, giving a marked precipitate of different lengths towards the anode. All the O antigens analyzed with the exception of O4 were nonmobile and gave a precipitate next to the start basin. The O4 precipitated towards the anode. These data indicate a possibility for using counterimmunoelectrophoresis in the diagnosis of Klebsiella antigen in body fluids or for capsular typing of the bacteria using the same technique.

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