Immunodiagnostic usefulness of monoclonal antibodies specific to conformational epitopes of Taenia solium oncosphere protein TSOL18

Emmanuel Assana,André Pagnah Zoli,Charles G Gauci,Marshall W Lightowlers,Pierre Dorny

doi:10.1016/j.jim.2021.113121

Abstract

Taenia solium oncosphere protein TSOL18 is the host-protective antigen against porcine cysticercosis. Little attention has been given to use it as target molecule in immunodiagnostic tests. The objective of this paper is to describe the immunodiagnostic potential of monoclonal antibodies (MoAbs) raised against conformational epitopes of TSOL18. Three murine IgG1 MoAbs (25D12C1, 21C2D2, 10H1F2) against three different conformational epitopes of TSOL18 were produced and evaluated with an inhibition enzyme-linked immunosorbent assay (i-ELISA) for the detection of anti-TSOL18 and anti-oncosphere antibodies. Serum samples from pigs immunized with TSOL18 inhibited the binding of the three MoAbs to TSOL18 antigen in i-ELISA. The highest inhibition of anti-TSOL18 antibodies in immunized pigs was observed with MoAb 25D12C1. Ten field sera (12.19%) from 82 non-vaccinated and non-infected pigs showed anti-oncosphere antibodies inhibiting the binding of MoAb 25D12C1. Anti-oncosphere antibodies in pigs experimentally infected with T. solium eggs inhibited the binding of MoAb 25D12C1 from 2 to 8 week-post infection. It is concluded that MoAb 25D12C1 has excellent immunodiagnostic potentials to detect anti-oncosphere antibodies in the intermediate hosts at early exposure to T. solium eggs. Further investigations on potential use of MoAb 25D12C1 in a capture antigen ELISA for the detection of post-oncospheral antigens in infected pigs cannot be overemphasized.

Highlights

Taenia solium taeniasis/cysticercosis has a complex two-host life cycle with pigs as intermediate hosts harboring the larval stages or metacestodes and humans as both aberrant intermediate hosts and definitive hosts harboring the adult tapeworm in the small intestine
Immunodiagnostic tool using monoclonal antibodies against conformational epitopes of TSOL18 to detect anti- oncospheral anti bodies or oncospheral antigen and not cysticercal antigens would be of interest in epidemiological studies, for the detection of pigs with exposure to T. solium eggs
This study was carried out to evaluate the efficiency of murine monoclonal antibodies (MoAbs) against conformational epitopes of TSOL18 to inhibit the binding of antioncosphere antibodies to TSOL18 in inhibition enzyme-linked immunosorbent assay (i-ELISA) using serum sample from

Summary

Introduction

Taenia solium taeniasis/cysticercosis has a complex two-host life cycle with pigs as intermediate hosts harboring the larval stages or metacestodes and humans as both aberrant intermediate hosts and definitive hosts harboring the adult tapeworm in the small intestine. The antigen is expressed at the surface of hatched oncospheres and not in the adult tapeworm or in fully devel oped metacestodes, indicating that it is a stage-specific antigen (Gauci et al, 2006; Kyngdon et al, 2006; Matinez-Ocana et al, 2011) This suggests that postoncospheral antigens excreted at early exposure of pigs to T. solium eggs can be detected using anti-TSOL18 antibodies. Immunodiagnostic tool using monoclonal antibodies against conformational epitopes of TSOL18 to detect anti- oncospheral anti bodies or oncospheral antigen and not cysticercal antigens would be of interest in epidemiological studies, for the detection of pigs (and humans) with exposure to T. solium eggs. This study was carried out to evaluate the efficiency of murine MoAbs against conformational epitopes of TSOL18 to inhibit the binding of antioncosphere antibodies to TSOL18 in i-ELISA using serum sample from

Objectives

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Results