Abstract
Objective To develop a prompt and specific method for diagnosis of lung cancer. Methods A murine monoclonal antibody against lung cancer cells was developed and characterized with the techniques of ELISA, immunohistochemistry and immunocytochemical detection of sputum. Results The antibody selectively bound to lung cancer tissues and exfoliated cells in the sputum from the patients with lung cancer , but did not bind to normal lung tissues and the cells in sputum either from the patients with pneumonia or from normal individuals. Conclusion The antibody 2C2S has potential application for immunocytological detection of lung cancer cells in sputum.
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