Immunodetection of C-met-Oncogene's Protein Product in Renal Cell Neoplasia

Abstract

The proto-oncogene c-met encodes a transmembrane tyrosine kinase receptor for hepatocyte growth factor and is expressed in normal kidney tissue. This receptor may be involved in the carcinogenesis of various organs. The aim of this study was to investigate the relationship between c-met immunohistochemical expression and pathological tumor variables in human renal cell carcinomas (RCCs) and adenomas (RAs). Formalin fixed, paraffin embedded tissues from 35 RCCs, 16 RAs and 17 normal kidneys were immunostained (Strept. ABC/HRP) with a polyclonal antibody against c-met protein (Santa Cruz, Clone C-12). The statistical analysis was performed using the linear trend in proportions and Fisher's exact test. C-met protein was detected in the cytoplasm and the plasma membranes of neoplastic cells in 19/35 RCCs (54%), 10/16 adenomas (63%) (p = 0.41) and in 17/17 controls in the epithelial cells of distal renal tubules and collecting ducts. C-met protein expression was not related with gender (p = 0.45), age (p = 0.18), tumor size (p = 0.99), cell type (p = 0.26), grade (p = 0.86) and stage (p = 0.33). Moreover, c-met immunopositive tumor cell percentage and intensity were increased in stage [RCCs, but these results were not statistically significant. Apart from glandular differentiation, c-met protein expression was not related to other histopathological features (i.e. to the type of cells or to any selective overexpression in tumor cells). C-met product may be involved in the malignant transformation of tubular epithelial cells as an early event in RCC carcinogenesis. C-met expression does not seem to have any prognostic significance for RCCs, as it was not associated with any pathological prognosticator.