Immunodeficient mice balb/c nude and modeling of various types of tumor growth for preclinical studies

H M Treshalina

doi:10.17650/1726-9784-2017-16-3-6-13

Abstract

Obtaining preclinical models of malignant growth in a variety of xenografts (heterotransplantation), usually the subcutaneous, is the most important area for the use of nude mice in oncology. In a review paper describes the characteristics of Balb/c nude mice obtained from Buffalo (USA) and introduced to the Federal State Budgetary Scientific Institution “N.N. Blokhin Russian Cancer Research Center» of the Russian Ministry of Health of the Russian Federation and systematized their application. There are various variants «palentology» models of tumor growth, allowing you to personalize the forecast performance of selected impacts for a particular patient. Detailed modeling of metastases of various human cancers: colon cancer, pancreas, esophagus, lung, breast and prostate glands, testicles and ovaries, tumors of the head and neck. The characteristic of orthotopic (“MetaMouse” model) and experimental confirmation of the value of the hypothesis of Paget’s “seed and soil”, postulating a paired part of the process of metastasis of cell and tissue components of the tumor. Describes the use of the subcutaneous xenografts as control cell lines in the study of oncogenic potencies of the various drugs that are recommended for cellular immunotherapy of human. Examples and main thematic literature in recent years.

Highlights

Obtaining preclinical models of malignant growth in a variety of xenografts, usually the subcutaneous, is the most important area for the use of nude mice in oncology
In a review paper describes the characteristics of Balb/c nude mice obtained from Buffalo (USA) and introduced to the Federal State Budgetary Scientific Institution “N. N
Describes the use of the subcutaneous xenografts as control cell lines in the study of oncogenic potencies of the various drugs that are recommended for cellular immunotherapy of human

Summary

Introduction

Obtaining preclinical models of malignant growth in a variety of xenografts (heterotransplantation), usually the subcutaneous, is the most important area for the use of nude mice in oncology. Доклинические модели метастазов опухолей человека Моделирование метастазов рака кишечника Эксперименты выполняют методом имплантации операционного опухолевого материала прямо от пациента (хирургическая ортотопическая имплантация, surgical orthotopic implantation, SOI) в толстую или слепую кишку nude-мышей. Что метастатический потенциал ортотопически развившейся опухоли выше, если для инокуляции использовали фрагменты опухолевой ткани, а не взвесь опухолевых клеток с дополнительной гистологической верификацией процесса.

Results

Conclusion