Immunocytological and FISH analysis of pole cell formation and soma elimination of germ line-limited chromosomes in the chironomid Acricotopus lucidus.

Abstract

In the chironomid Acricotopus lucidus, germ line-soma differentiation becomes evident with the formation of the pole cells and the elimination of the germ line-limited chromosomes (Ks) from the future somatic nuclei of the embryo. Unlike in Drosophila, the early nuclear divisions do not proceed synchronously in A. lucidus. Usually, only one nucleus, the future pole nucleus, penetrates into the pole plasm, always at a telophase stage in the course of a regular mitosis. This happens by chance, depending on the orientation of the mitotic spindles of the early syncytial nuclei. Consequently, the time and the cell cycle at which a nucleus reaches the pole plasm, and pole cells arise, vary between embryos of the same oviposition. When entering the first germ line mitosis, while polar plasm and syncytial plasm are still not separated, some future somatic nuclei begin to eliminate their Ks. While the soma chromosomes (Ss) undergo normal anaphasic migration to the opposite poles, the K chromatids do not separate and remain in the equatorial plane, as demonstrated by fluorescence in situ hybridization using germ line-specific DNA probes. The elimination of the Ks does not occur at the same time in all future somatic nuclei. Nondisjunction of Ks was observed in the first mitosis of the pole nucleus, leading to primordial germ cells with different compositions of their K complements. The pattern and timing of elimination mitoses in the embryos indicate that each of the future somatic nuclei seems to regulate the elimination of the Ks autonomously.