Immunocytochemical localization of tissue-bound oestradiol in rat paracervical ganglion.

Abstract

The uterine paracervical ganglion (Frankenhauser's ganglion) contains the terminal neurons of the cholinergic sacral parasympathetic, the short adrenergic sympathetic and the peptidergic (vasoactive intestinal polypeptide-containing) nerves of the internal genitalia. Previous studies have shown that either the number of cells or transmitter content of each of these neuronal systems is altered by variations in steroid hormones. Furthermore, our recent study showed that some component of the rat paracervical ganglion was capable of metabolizing [3H]oestradiol to oestrone and the 2-OH and 4-OH forms of oestrone and oestradiol. The present study employs the peroxidase-anti-peroxidase immunohistochemical method to localize oestradiol in rat paracervical ganglia. Specific reaction product was identified in (1) cytoplasm and some nuclei of principal ganglion cells, (2) cytoplasm of large vacuolated ganglion cells, (3) cytoplasm of 'small intensely fluorescent' cells and (4) some nerve fibres in ganglia from animals in oestrus. The cytoplasm of principal neurons and some nerve fibres exhibited specific staining for oestradiol in dioestrus and pro-oestrus. No oestradiol was localized in ganglia excised from animals in metoestrus. Preincubation in oestradiol before fixation was necessary for specific localization of oestradiol; treatment of tissues with oestradiol after fixation was not required. These results are not consistent with binding of oestradiol to the classical oestrogen receptor. The resistance of oestradiol to organic solvent extraction suggests that oestradiol is covalently bound to tissue proteins. Such covalently bound oestradiol has been reported as a by-product of tissue metabolism of oestradiol via P-450 enzymes.