Immunocytochemical localization of the neurons in the superior mesenteric ganglion innervating the small intestine of the cat

Abstract

Retrograde tracing was used to determine the localization of neuronal perikarya and fibres in the feline superior mesenteric ganglion (SMG), projecting to the small intestine. In the distal part of the ileum, a retrograde neuronal tracer Fast Blue (FB) was injected and after approximately thirty five to forty days the animals were killed by perfusion. The SMG were removed and the neuropeptide contents of the neurons, projecting to the distal ileum, were determined by means of immunofluorescence with antisera to neuropeptide Y (NPY), calcitonin gene-related peptide (CGRP), substance P (SP), somatostatin (SOM), and vasoactive intestinal polypeptide (VIP). Neurons innervating the small intestine were located in the upper part of the SMG and all of them were NPY-immunopositive. The group of CGRP-immunoreactive (IR) cells was less numerous (73.33%). Probably the FB-labeled fibres, containing the same neuropeptides, arise from these perikarya. SP- or VIP-immunopositive neuronal processes were found to surround immunonegative ganglionic cells but their origin is not in the ganglion. Only single FB-marked cells were VIP-immunopositive. SP- and SOM-immunoreactive amounted respectively to 2.28% and 3.01% of all the neuronal population, but only a few of these cells were FB-labelled.