Immunocytochemical localization of gamma-aminobutyric acid in the hypoglossal nucleus of the macaque monkey, Macaca fuscata: a light and electron microscopic study.

Abstract

The hypoglossal nucleus of the macaque monkey Macaca fuscata was investigated with light and electron microscopic immunocytochemistry with an antibody directed against gamma-aminobutyric acid (GABA). At the light microscopic level, GABA immunoreactivity was present in small neurons, punctate structures, and thin, fiberlike structures. These GABA-positive elements were distributed throughout the hypoglossal nucleus at rostrocaudal levels. There was no immunoreactivity in the hypoglossal motoneurons. The GABA-positive small neurons were fusiform or ovoid (15 X 9 micron) and extended a few proximal dendrites from both poles. At the ultrastructural level, these small neurons were characterized by a markedly invaginated nucleus and a scanty cytoplasm in which cisternae of rough endoplasmic reticulum were not organized into extensive lamellar arrays as seen in the motorneurons. The GABA-positive punctate structures at the light microscopic level were identified as vesicle-containing axon boutons at the electron microscopic level. These GABA-positive axon terminals made synaptic contacts mainly with the dendrites of the motoneurons and infrequently with the somata. The majority of them made symmetric synapses and they contained pleomorphic synaptic vesicles. However, a small number of GABA-positive terminals (7%) formed asymmetric synapses with the dendrites of motoneurons, and these contacts exhibited postsynaptic dense bars or Taxi bodies lying beneath the postsynaptic membranes. There were no GABA-positive boutons that contacted the cell bodies of the small neurons. Although GABA-positive myelinated and unmyelinated axons were seen as thin, fiberlike structures, these myelinated and unmyelinated axons rarely gave rise to boutons on the motoneurons. The present study suggests that GABAergic inhibition in the monkey hypoglossal nucleus occurs mainly on the dendrites of the motoneurons and to some extent on the somata.