Immunocytochemical evidence for the colocalization of neurotensin/xenopsin- and gastrin/caerulein-immunoreactive substances in Xenopus laevis gastrointestinal tract

Abstract

Distribution and association of neurotensin (NT)- and xenopsin (XP)-like peptides were investigated using immunocytochemical techniques in the amphibian gut. Antisera against both groups of peptides showed an identical distribution pattern of NT- and XP-positive cells in Xenopus laevis gastrointestinal tract. Immunolabeling of consecutive semithin sections revealed the coexistence of NT- and XP-like substances within cells of the stomach and small intestine. Recent reports of the colocalization of XP-like material with gastrin in mammalian G cells led us to study the association of NT/XP-like peptides with members of the gastrin/cholecytokinin (CCK)/caerulein (G/C) family in amphibians. The data obtained from immunolabeling serial sections with NT/XP-specific and G/C-specific antisera show that in some intestine NT/XP- and G/C-like peptides do exist in the same cells. In the stomach, however, G/C-like material is confined to endocrine cells of the antral region, while NT/XP-like substances occur in distinct cells accumulating in cardial glands but absent in the pyloric glands. Our findings thus indicate that in amphibian gastrointestinal tract there is some association between the regulatory peptide families NT/XP and G/C, similar to mammals. The regional distribution of both hormone families, however, is different from that in mammals.