Immunochromatographic strip for rapid detection of phenylethanolamine A

Wei Jiang,Lu Zeng,Liqiang Liu,Shanshan Song,Hua Kuang

doi:10.1080/09540105.2017.1364709

Wei Jiang, Lu Zeng + Show 3 more

Open Access

https://doi.org/10.1080/09540105.2017.1364709

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Abstract

ABSTRACT A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.

Highlights

Phenylethanolamine A (PEA, 2-[4-(nitrophenyl) butan-2-ylamino]-1-[4-methoxyphenyl]ethanol, C19H24N2O4) has been used illegally in animal husbandry in China in recent years (Tang, Cai, Deng, & Li, 2015)
Enzyme-immunoassay-grade horseradish peroxidase (HRP), HRP-labeled goat antimouse immunoglobulin G (IgG) antibody, 3,3′,5,5′-tetramethylbenzidine (TMB), and gelatin were from Sigma-Aldrich
Bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), and OVA are commonly used for this purpose

Summary

Introduction

Phenylethanolamine A (PEA, 2-[4-(nitrophenyl) butan-2-ylamino]-1-[4-methoxyphenyl]ethanol, C19H24N2O4) has been used illegally in animal husbandry in China in recent years (Tang, Cai, Deng, & Li, 2015). Instrumental detection methods are characteristically highly accurate (Tang et al, 2015), the instruments are expensive, the preprocessing methods and operations are complex, and the times required for detection are long. These properties limit the use of these techniques for rapid screening. The selectivity and sensitivity of immunoassays are much higher than those of conventional physical and chemical analyses. They are applicable to the analysis of trace components in complex samples (Dai et al, 2015)

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