Immunochromatographic direct on sampling filter test for aeroallergens

Abstract

An immunochromatographic method for qualitative and quantitative determination of aeroallergens direct on sampling (ADOS) filters has been developed. In this method, a porous polytetrafluoroethylene filter carrying adsorbed allergens is fixed by double-coated adhesive tape to a supporting filter paper matrix. Following addition of antibodies specific for the relevant allergens and washing and staining reagents via a reagent applicator an immunochromatogram is developed resulting in a 5–10 mm wide area of the sample filter covered with blue-violet-stained spots appearing on a faintly pink or white background. The method takes 30 to 90 min, depending on the nominal porosity (1.2–5 μm) and the defined reaction area (5–10 mm) of the sample filter. Application experiments with birch and grass pollen, soluble Bet v 1, Phl p 5 and mould allergens as well as cat allergen carried by airborne dust revealed a limit of detection of a few picograms of allergen as stained spots. The specificity of the new method to evaluate the type of allergen is a function of the selected antibodies. The concentrations of the allergen in an air sample are related to the number and intensity of stained spots.