Abstract
This chapter discusses immunochemistry of cattle blood group J hapten. Cattle J, sheep R, pig A, and human Lewis blood group substances are synthesized in cells other than the red cells and are secreted into the plasma. Red cells can absorb these substances from the plasma and acquire the respective blood group character of the absorbed substance. It has been demonstrated that the Lewis substances in plasma and in red cells are glycolipids, and preliminary reports have claimed that the cattle J hapten is extractable from plasma by a mixture of chloroform and methanol. The chapter presents a procedure for the isolation of the blood group J hapten from cattle serum and describes its composition and some of its properties. Purified blood group J hapten is a glycolipid that contains glucose, galactose, a long chain base, palmitic acid, glycerol, and phosphorus. Mixtures of cholesterol and lecithin do not potentiate the activity of the purified J hapten. The A active glycoproteins contain both 2-acetamido-2-deoxy-D-galactose and 2-acetamido-2-deoxy-D-glucose. Both of these sugars are absent from the J hapten.
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