Abstract
From a feline patient, monoclonal-IgA (M-IgA) and Bence Jones (B-J) proteins were isolated and charaterized for their biochemical properties. The molecular weight of the purified M-IgA was estimated to be approximately 230, 000 daltons and the molecular weights of the B-J proteins were estimated to be 28, 500 and 25, 000 daltons, by SDS-polyacrylamide gel electrophoresis. The biochemical and immunochemical data support our earlier conclusion that the feline patients suffered from an M-IgA gammopathy associated with B-J proteinuria. Furthermore, B-J proteins in the patient's urine appeared to contain at least five B-J protein components, when analyzed by two-dimensional gel electrophoresis. These M-IgA and B-J have common antigenic determinants and identical polypeptide segment, as demonstrated by immunological tests and by the analysis of amino acid composition. The analysis of the amino acid composition of the feline B-J proteins also indicated that it is similar to that of the human B-J protein.
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