Abstract
Abstract Two different immunoassay (IA) formats for pesticide residue analysis, a field format (dipstick IA) and an automated version (flow injection immunoanalysis, FIIA), are described with regard to method development and validation performed by measuring water, soil, and liquid food samples and comparing the results with the respective GC data obtained. As representative analyte a pesticide metabolite, deethylatrazine as the major atrazine metabolite, was selected. The measuring range for deethylatrazine was from 0.1–10 μg/L with the dipstick IA and from 0.01–10 μg/L with the FIIA. With both formats, water and liquid food samples can be measured directly without the need for any prior enrichment or clean-up steps. In the case of the soil samples, two extraction steps can be omitted as compared to extract preparation for GC analysis which usually takes further 3–4 h. A close correspondence was found between the results of the two immunoassay formats and GC measurements.
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