Abstract

Rabbit antibodies were prepared against glycerol 3-phosphate dehydrogenase (EC 1.1.1.8) purified from the flight muscle of honeybees, one bumblebee species, and yellow jackets. Crossreactivity tests by means of quantitative microcomplement fixation were conducted with extracts from several hymenopteran species. The degree of cross-reaction with thoracic extracts was, in general, compatible with the accepted phylogenetic relationships of the species tested. The one exception was the lower-than-expected reaction of the honeybee enzyme with anti-bumblebee glycerol 3-phosphate dehydrogenase. The cross-reactivity of the two types of isozymes of bumblebee glycerol 3-phosphate dehydrogenase was measured. The alarmuscular variants, which occur mainly in flight muscle, reacted much more strongly with anti-honeybee or anti-bumblebee sera than did the omniregional variants.

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