Abstract
Blood group substance was isolated from ovarian cyst fluid of a Nigerian, Tij, by digestion with pepsin, precipitation by ethanol, solution in 90% phenol, and fractional precipitation with ethanol from phenol. There was a progressive decrease in fucose and galactose and in B activity and an increase in I activity of group 3 (anti-I Step) and i activity in fractions requiring more ethanol for precipitation from phenol, indicating that the carbohydrate chains were becoming smaller. I activity of group 1 (anti-I Ma) tended to be higher in the fractions precipitated at lower concentrations of ethanol. One of the fractions showed high reactivity toward concanavalin A.
Published Version
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