Abstract
Binding experiments with tobacco mosaic virus and IgG antibody were analysed by two types of Scatchard plot. In a large antibody excess the IgG molecules were bound univalently and the antigenic valence of the virus was 780.At lower antibody-antigen ratios, IgG was bound in monogamous bivalent fashion and the same antigenic valence (800) as previously observed with specific Fab fragments was found. The biphasic nature of the curves was considered to be due to a change-over from univalent to bivalent binding. Association constants calculated in the region where IgG binds univalently were considerably lower than for bivalent binding.
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