Abstract
A neutral glycosphingolipid containing fatty acid-sphingosine (long chain base)-glucose-galactose-galactosamine in the molar proportions 1:1:1:2:1 has been isolated from normal human kidney by successive chromatographic separations on silicic acid, magnesium silicate, and silicic acid. The isolated substance, designated cytolipin K, was shown to have haptenic properties by screening it for reactivity with a large number of rabbit antisera prepared against diverse human tissues. Good reactions were observed in a small number of instances. The two most reactive antisera were obtained by combination immunization with lipids from a multiple myeloma-affected kidney and a pyelonephritic kidney, and with these antisera and optimal addition of auxiliary lipid, 0.003–0.005 μg. of cytolipin K was readily measured. It was shown that the immunological reactivity of cytolipin K was distinct with respect to five other lipid haptens present in human tissues, namely, cytolipin H, cytolipin G, galactocerebroside, cardiolipin, and Forssman hapten.
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