Abstract
Two immunoassays for prostaglandins are described: radioimmunoassay and “chemically modified bacteriophage” technique. Prostaglandin F 2α was attached to bacteriophage T 4 via a carbodiimide derivative. The surviving phage population could be inactivated with antibodies prepared in goat against a bovine serum albumin conjugate of prostaglandin F 2α. The inactivation could be inhibited to a different extent by various prostaglandins. The sensitivity of both assays was high and similar (0.45 and 0.75 nanograms of PGF 2α for “chemically modified bacteriophage” technique and radioimmunoassay, respectively). Using 25% inhibition of the modified phage inactivation, as little as 25 picograms of PGF 2α could be detected.
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