Immunochemical characterization of binding sites of hybridoma antibodies specific for α(1 → 6) linked dextran

Abstract

The combining sites of seven BALB/c IgM, four BALB/c IgA and one C57BL/6 IgA hybridoma antibodies specific for α(1 → 6) linked dextran were probed by precipitin and precipitin inhibition assays. The 12 antibodies are able to bind to linear determinants in the interior of the dextran molecule; some have sites complementary to six α(1 → 6) linked glucose residues and others have sites complementary to seven α(1 → 6) linked glucose residues. From the analysis of the precipitins and precipitin inhibitions, it is concluded that no two hybridoma proteins have identical binding sites.