Abstract
The use of specific antibodies to each of the two isoenzymes of carbonic anhydrase (CA I and CA II) shows that only CA II is intrinsic to nervous tissue, and all CA I detected is due to blood contamination. CA II is present in the cytosol as well as in the insoluble fraction of cerebellar homogenates. A small portion of the enzyme present in the sediment is a ‘cytosol’ enzyme trapped in membrane-limited structures but the majority appears to be membrane-bound. Immunohistology of CA II at the light and electron microscope levels and the use of double labelling technique with an astrocyte marker (glial fibrillary acidic protein) show that CA II is exclusively localized in cells with oligodendrocyte morphology and in their processes. Myelin appears to contain CA II only in the most peripheral lamella. Astrocytes, neurons and cells of capillary walls appear not to contain CA II. It can be concluded that carbonic anhydrase II serves as a marker for oligodendrocytes.
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