Immunochemical and immunohistochemical determination of ubiquitin in molluscan ( Mytilus edulis) smooth muscle

Abstract

1. 1. Immunochemical and immunohistochemical distribution of ubiquitin in the anterior byssus retractor muscle (ABRM) of Mytilus edulis was investigated. 2. 2. In immunostaining, specific ubiquitin immunoreactivity was observed in the cross-sectioned ABRM, and was uniformly localized in this section. 3. 3. The amount of free ubiquitin in the ABRM homogenate was 130 ± 4.6 ng/mg protein by western blot analysis, and ubiquitin conjugates were found at about 25, 29 and 200–230 kDa. 4. 4. These findings were similar to those obtained in the skeletal muscle of rat.