Abstract
Phosphate buffer extracts (pH 8.0) of the brain and the spinal cord of various vertebrates, mammalian and submammalian, cross-reacted with antihuman glial fibrillary acidic (GFA) protein serum by Ouchterlony double diffusion. Mammalian brain extracts, as previously reported, gave an immunodiffusion pattern of complete identity with human GFA protein. In submammalian vertebrates (shark, goldfish, turtle and chicken) strong spurs were observed at the junction with human GFA protein, suggesting incomplete identity. GFA protein from mammalian and submammalian vertebrates had the same extraction properties, immunoelectrophoretic mobility and molecular weight as human GFA protein. By immunofluorescence with antihuman GFA protein, serum astrocytes were selectively stained in cryostat sections of the brain and the spinal cord in all vertebrates studied, mammalian and submammalian, with the exception of the lamprey. No immunofluorescence was observed in invertebrate ganglia. Some antomical observations on the astrocytic framework of various vertebrates, as observed with immunofluorescence, are reported.
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