Abstract
Abstract 1. 1. Immunofluorescent methods have been used to detect myosin in the A bands of skeletal muscle, not only in intraspecific systems (immunizing antigen and stained muscle from the same species), but in interspecific systems (immunizing antigen and stained muscle from different species) as well. 2. 2. Two recent observations suggested that the interspecific staining reactions might be spurious: myosins purified by several procedures have been shown to contain antigenically active contaminants; and positive intraspecific reactions between anti-skeletal muscle myosin and cardiac myosin do not occur in rigorously purified systems. 3. 3. The test the possibility that interspecific reactions might be due to the presence of antigenically active contaminants, rabbit and chicken skeletal muscle myosins of various degrees of purity and their antisera were evaluated by gel-diffusion procedures. The cross reactions observed ranged from marked to absent depending upon the relative purity of the systems, thus demonstrating that adequately purified chicken and rabbit skeletal muscle myosins are completely dissimilar from one another by immunochemical criteria.
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