Abstract
Polyvalent, equine antitoxin (250 IU/mouse) passively protected NMRI mice against 7 of 9 challenge strains of Clostridium perfringens type A. Two human immunoglobulin G (IgG) preparations (Polyglobin N and Sandoglobulin) and two rabbit immune sera prepared against formalinized cells of C. perfringens were ineffective. Cell homogenates of 4 C. perfringens strains revealed more than 15 polypeptides in SDS-PAGE electropherograms; the molecular weights ranged from 138 to 14.4 kD. Normal human serum from 2 donors revealed up to 3 immunoblot-reactive antibodies against 126-, 69.2-, 49- or 14.4-kD antigens, the two rabbit immune sera showed antibodies against 4-7 distinct antigens that ranged from 138 to 28.8 kD, whereas the two human IgG preparations revealed antibodies against up to 12 antigens (range 138.7-31.6 kD) and antibodies against lower-molecular-weight (less than 24.5-kD) components. Polyvalent, equine antitoxin reacted with at least 14 polypeptides (range 136.5-31 kD) and numerous smaller (less than 24-kD) components.
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