Abstract
Parasites have been encapsulated within sol-gel silica matrices. Thermoporometry measurements show that the pore size depends strongly on experimental conditions. Pores as large as 100 A in diameter can be obtained, allowing the diffusion of large biomolecules such as immunoglobulins. TEM observations, performed on procaryote (bacteria) and eucaryote (protozoa) specimen show that the cellular organization and the integrity of the plasma membrane of entrapped parasites are preserved. Moreover they retain their antigenic activity and can react specifically with the corresponding antibodies. Sol-gel matrices have then been used for the realization of enzyme-linked immunosorbent assays (ELISA) directly with the sera of infected patients. Two examples are described, one with parasitic protozoa, Leishmania, and the other one with the cystic hydatid stage of tapeworm parasites, Echinococcus granulosus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
