Abstract
ABSTRACTThe United States Department of Agriculture (USDA) requires that meat and poultry products be processed to specific minimum endpoint temperatures (EPT) to ensure destruction of pathogens. Several enzyme‐linked immunosorbent assays (ELISA), using antibodies that recognize different indicator proteins, were developed to verify minimum EPT in several products. These assays are highly specific and can be used by minimally trained personnel to rapidly verify EPT without the need for separation steps or sophisticated scientific equipment necessary with other EPT assays. A sandwich ELISA, using lactate dehydrogenase (LDH) as the marker protein, was used to verify EPT of turkey breast rolls and turkey ham. Two other indirect competitive ELISAs, detecting turkey serum albumin and Immunoglobulin G, were compared for their ability to verify minimum EPT in turkey ham. Self‐contained ELISA field kits can be devised to verify compliance to USDA minimum temperatures in about 30 min or less.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
