Immunoassay Using Chemiluminescence Detection of Dyestuff-Containing Liposomes as a Labeling Reagent

Abstract

A new immunoassay using chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent is proposed. Human serum albumin labeled with Eosin Y containing-liposomes was employed as a labeling reagent for an immunoassay. After the immune reaction was carried out in the presence of an antibody-immobilized glass bead, the reactant solution was subjected to the capillary electrophoresis–chemiluminescence detection system. That is, the bound/free separation was conducted by use of glass beads, also, other coexisting compounds which might influence chemiluminescence detection were easily separated from the labeled human serum albumin by capillary electrophoresis. The labeled human serum albumin in the reactant solution was detected with high sensitivity. The amount of the labeled human serum albumin showed a good relationship to that of human serum albumin as an analyte through immune reaction. Human serum albumin could be determined over the range of 1 × 10−6 − 5 × 10−4 M. The present method was also applicable to the determination of protein in a serum sample without being interfered with by coexisting constituents.