Abstract
Most of the enzyme-immunoassays described in the literature employ enzyme-labelled antigen or hapten [l-8]. A few use enzyme-labelled anti-antibody (anti-IgG*) [8,9]. Enzyme-labelled antibodies against the substance to be determined, provide a third tool for enzyme-immunoassay. In this report we describe two methods for the assay of human chorionic gonadotrophin (HCG), which employ anti-HCG labelled with horse-radish peroxidase (HRP). The methods are analogous to the ‘immunoradiometric’ [lo] and the ‘sandwich’ [ 1 I] radioimmunoassays. Their sensitivities and the economy of reagents are compared with those of the Dasp enzyme-immunoassay for HCG, which employs HRPlabelled HCG. The latter method has been shown to be markedly more sensitive than the corresponding solid-phase assay [ 11.
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