Abstract
This report reviews the current status of immunoassays for circulating enzymes. The necessity for measuring the protein concentration of circulating enzymes is evident in the light of the inability of conventional enzyme assays to measure the levels of released enzymes accurately, especially when these enzymes are inactivated, bound to an inhibitor, or present as proenzymes. In some cases, therefore, measurement of enzyme protein concentrations rather than their catalytic activities provide more relevant information for detection of lesions and evaluation of their extent in the organs from which the enzymes are derived. Facts to be considered in the measurement and interpretation of the values obtained by immunoassays are discussed.
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