Abstract
A specific radioimmunoassay for avian pancreatic polypeptide (APP) is described. At an antiserum dilution of 1:50,000 the assay is usable over a range of 0.02 to 1.25 ng APP per tube. At the lowest level of standard, cumulative within-assay variance at the 95% confidence level was ±6.58% and at the highest level was ±16.68%. Cumulative between-assay variance ±2.7 to ±5.0% over the same range. Fifty percent displacement of 125I-APP occurred at 0.45 ng APP per tube. Recovery of APP added to chicken plasma was ±5.0% except at levels near 20 ng/ml where estimates of APP content were 10–15% high. Dilution curves paralleled the standard curves except at high APP content. The immunoassay detected APP in acid alcohol extracts of chicken pancreas and duodenum. No APP could be detected in other organs. The disappearance from plasma of APP injected intravenously into chickens could be fitted to a two-compartment model with T 1 2 values of 2.45 and 8.28 min, respectively.
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