Abstract
Sample preparations are crucial to the success of all analytical methods. However, sample preparation is often labor-intensive and the rate limiting step of the overall analytical scheme. Certain immunochemical procedures can perform difficult separations, making subsequent analysis simpler and ensuring the specificity of the results. Immunoaffinity chromatography (IAC) is based on the ability of antibodies to extract target analytes, even from complex environmental or biological matrices. High-performance liquid chromatography-mass spectrometry (HPLC/MS) can be coupled to IAC for a complete extraction-detection system. Customized IACs for plasma, urine, water, and milk are discussed in this paper. Portable detection methods which may be suitable to site characterization are described, including capillary electrochromatography coupled with laser-induced fluorescence (CEC/LIF) detection.
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